ToolJovia
Data

Bounded FASTQ Quality Profiler

Measure a declared four-line Phred+33 DNA FASTQ profile without guessing encoding or changing bases.

✓ Browser-local processing
Files are read locally and are not uploaded.
Processed in this tab. Up to 100,000 characters unless this tool states a smaller limit.

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How to use Bounded FASTQ Quality Profiler

Inspect read lengths, per-position quality, quality histograms and supplied error-probability summaries locally. The report distinguishes mean Phred from the Phred transform of mean estimated error probability and keeps unavailable certainty out of the result.

  1. Enter optional local utf-8 source file, input.
  2. Choose the settings for bounded fastq quality profiler, then run the tool.
  3. Review the result and any limitations before copying or downloading it.

Good to know

  • 200,000 UTF-8 source bytes/characters, 5,000 unwrapped four-line reads, 1,000 bases per read and 80,000 bases total. File input takes precedence.
  • Only A/C/G/T/N and Phred+33 printable quality characters are supported. Multiline FASTQ, Phred+64, Solexa encoding and inferred trimming are refused or unsupported. Read details show the first 1,000 reads; summary counts cover all accepted reads.
  • Processing is local. Output is limited to 1,000,000 text characters unless a stricter file limit is stated.

Common questions

Can the tool autodetect an older quality encoding?

No. Character ranges overlap between encodings. Use this only when Phred+33 is known, rather than treating an ambiguous guess as measured quality.

Is this input sent to a server?

No. The application processes this input inside your browser tab and does not save it to persistent storage.

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